Toll-Like Receptor 4 Triggering Promotes Cytosolic Routing of DC-SIGN-Targeted Antigens for Presentation on MHC Class I.
作者： Sophie K. Horrevorts , Sanne Duinkerken , Karien Bloem , Pablo Secades , Hakan Kalay
关键词: Cross-presentation 、 T cell 、 MHC class I 、 Cell biology 、 Chemistry 、 Toll-like receptor 、 DC-SIGN 、 CD8 、 Internalization 、 Antigen
摘要: DC-SIGN is an antigen uptake receptor expressed on dendritic cells (DCs) with specificity for glycans present a broad variety of pathogens and capable directing its cargo to MHC-I MHC-II pathways the induction CD8+ CD4+ T cell responses, respectively. Therefore, very promising target delivery anti-cancer vaccination. Although endocytic route leading presentation characterized large extent, mechanisms controlling targeted cross-presentation exogenous peptides MHC-I, are not completely resolved yet. In this paper, we used imaging flow cytometry antigen-specific investigate intracellular fate in human DCs. Our data demonstrates that immature DCs toll-like 4 (TLR4) stimulated had similar internalization capacity were both able cross-present via DC-SIGN. Interestingly, simultaneous triggering TLR4 resulted translocation cytosol, proteasome-dependent processing increased activation. Understanding dynamics DC-SIGN-mediated essential design optimal DC-SIGN-targeting vaccination strategies aimed at enhancing responses.
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