Extensive repertoire of membrane-bound and soluble dendritic cell-specific ICAM-3-grabbing nonintegrin 1 (DC-SIGN1) and DC-SIGN2 isoforms. Inter-individual variation in expression of DC-SIGN transcripts.

摘要: Expression in dendritic cells (DCs) of DC-SIGN, a type II membrane protein with C-type lectin ectodomain, is thought to play an important role establishing the initial contact between DCs and resting T cells. DC-SIGN also unique human immunodeficiency virus-1 (HIV-1) attachment factor promotes efficient infection trans that express CD4 chemokine receptors. We have identified another gene, designated here as DC-SIGN2, exhibits high sequence homology DC-SIGN. Here we demonstrate alternative splicing DC-SIGN1 (original version) DC-SIGN2 pre-mRNA generates large repertoire DC-SIGN-like transcripts are predicted encode membrane-associated soluble isoforms. The range mRNA expression was significantly broader than previously reported included THP-1 monocytic cells, placenta, peripheral blood mononuclear (PBMCs), there cell maturation/activation-induced differences levels. Immunostaining term placenta DC-SIGN1-specific antiserum showed expressed on endothelial CC receptor 5 (CCR5)-positive macrophage-like villi. not detectable PBMCs. In DCs, lower DC-SIGN1. Notably, significant inter-individual heterogeneity expressed. genes forDC-SIGN1, CD23, Type lectin, colocalize ∼85 kilobase pair region chromosome 19p13.3, forming cluster related undergo highly complex events. molecular diversity DC-SIGN-1 -2 reminiscent observed for certain other adhesive surface proteins involved cell-cell connectivity. generation this collection polymorphic variants exhibit variation levels has implications pathogenesis HIV-1 infection, well code required establish interactions antigen-presenting i.e. immunological synapse.