High-performance liquid chromatographic method for the analysis of anthraquinone glycosides and aglycones in madder root (Rubia tinctorum L.)
作者： Goverdina C.H. Derksen , Teris A. van Beek , Æde de Groot , Anthony Capelle
DOI: 10.1016/S0021-9673(98)00492-0
关键词: Column chromatography 、 Glycoside 、 Anthraquinone 、 Alizarin 、 Anthraquinones 、 High-performance liquid chromatography 、 Chromatography 、 Phthalic anhydride 、 Rubia tinctorum 、 Chemistry
摘要: A HPLC method has been developed for the simultaneous characterisation of anthraquinone glycosides and aglycones in extracts Rubia tinctorum L. The anthraquinones are separated on an end-capped C18-RP column with a water–acetonitrile gradient as eluent measured UV detection at 250 nm. With this lucidin primeveroside ruberythric acid lucidin, alizarin, purpurin, quinizarin 2,6-dihydroxyanthraquinone can be analysed. Lucidin which is not commercially available was synthesised starting from resorcinol phthalic anhydride. mixture were by droplet counter-current chromatography ascending flow chloroform–methanol–water eluents prior to their use standards.
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