Validation of 24-Hour Posaconazole and Voriconazole MIC Readings versus the CLSI 48-Hour Broth Microdilution Reference Method: Application of Epidemiological Cutoff Values to Results from a Global Candida Antifungal Surveillance Program

作者: M. A. Pfaller , L. B. Boyken , R. J. Hollis , J. Kroeger , S. A. Messer

DOI: 10.1128/JCM.02437-10

关键词: MicrobiologyAntifungalCutoffPosaconazoleCandida sppSerial dilutionVoriconazoleBroth microdilutionMedicine

摘要: We performed 24- and 48-h MIC determinations of posaconazole voriconazole against more than 16,000 clinical isolates Candida species. By using the epidemiological cutoff values (ECVs), categorical agreement between 24-h reference broth microdilution results ranged from 97.1% (C. parapsilosis voriconazole) to 99.8% krusei voriconazole), with 0.0 2.9% very major discrepancies (VMD). The essential (within 2 log2 dilutions) was 99.6% for both voriconazole. obtained after only 24 h incubation may be used determine susceptibilities spp. these important antifungal agents. applications ECVs this large collection suggests potential develop species-specific breakpoints

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