Hes1-deficient mice show precocious differentiation of Paneth cells in the small intestine
作者： Katsumasa Suzuki , Hirokazu Fukui , Takahisa Kayahara , Mitsutaka Sawada , Hiroshi Seno
DOI: 10.1016/J.BBRC.2004.12.174
关键词: HES1 、 Small intestine 、 Columnar Cell 、 Cryptdin 、 Molecular biology 、 Cellular differentiation 、 Crypt 、 Stem cell 、 Paneth cell 、 Biology 、 Immunology
摘要: We have previously shown that Hes1 is expressed both in putative epithelial stem cells just above Paneth and the crypt base columnar between cells, while completely absent cells. This study was undertaken to clarify role of cell differentiation, using Hes1-knockout (KO) newborn (P0) mice. Electron microscopy revealed premature appearance distinct containing cytoplasmic granules intervillous region Hes1-KO P0 mice, whereas those were wild-type (WT) In gene expressions cryptdins, exclusively present all enhanced compared with WT Immunohistochemistry demonstrated increased number lysozyme-positive cryptdin-4-positive small intestinal epithelium mice as Thus, appears an inhibitory differentiation intestine.
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