A fully integrated multi-column system for abundant protein depletion from serum/plasma
作者： Dariusz J. Janecki , Steven C Pomerantz , Eric J. Beil , Jennifer F. Nemeth
DOI: 10.1016/J.JCHROMB.2012.06.010
关键词: Protein depletion 、 Fraction (chemistry) 、 Column (data store) 、 Fractionation 、 Reproducibility 、 High-performance liquid chromatography 、 Autosampler 、 Serum plasma 、 Chemistry 、 Chromatography
摘要: Abstract This work details the transformation of a conventional HPLC system to low back pressure liquid chromatography set-up for automated serum/plasma depletion and fractionation. A Dionex U3000 was converted operation (125 psi max) by replacing all narrow-bore lines larger inner-diameter tubing. The configured use two immunoaffinity columns, first top 14 most abundant proteins (Seppro IgY14), then next 200–300 SuperMix). autosampler dual-purposed both injection fraction collection. Both flow-through SuperMix bound were collected in an fashion. Three samples could be depleted consecutively before required user intervention, up nine within 24 h period. study documents validation instrument performance with 90-patient sample set, demonstrating overall CVs 86 90 95% confidence intervals. Additionally, there excellent reproducibility same patient (biological replicates) across days.
sci-hub.se 参考文章(27)
Kalpana Rengarajan, Marc D. de Smet, Barbara Wiggert, Removal of Albumin from Multiple Human Serum Samples BioTechniques. ,vol. 20, pp. 30- 32 ,(1996) , 10.2144/96201BM05
James Martosella, Nina Zolotarjova, Hongbin Liu, Gordon Nicol, Barry E. Boyes, Reversed-phase high-performance liquid chromatographic prefractionation of immunodepleted human serum proteins to enhance mass spectrometry identification of lower-abundant proteins. Journal of Proteome Research. ,vol. 4, pp. 1522- 1537 ,(2005) , 10.1021/PR050088L
Cinzia Magagnotti, Isabella Fermo, Rose Mary Carletti, Maurizio Ferrari, Angela Bachi, Comparison of different depletion strategies for improving resolution of the human urine proteome. Clinical Chemistry and Laboratory Medicine. ,vol. 48, pp. 531- 535 ,(2010) , 10.1515/CCLM.2010.109
Stéphane Roche, Laurent Tiers, Monique Provansal, Martial Seveno, Marie-Thérèse Piva, Patrick Jouin, Sylvain Lehmann, Depletion of one, six, twelve or twenty major blood proteins before proteomic analysis: the more the better? Journal of Proteomics. ,vol. 72, pp. 945- 951 ,(2009) , 10.1016/J.JPROT.2009.03.008
Thomas Linke, Sundari Doraiswamy, Earl H. Harrison, Rat plasma proteomics : Effects of abundant protein depletion on proteomic analysis Journal of Chromatography B. ,vol. 849, pp. 273- 281 ,(2007) , 10.1016/J.JCHROMB.2006.11.051
Chengjian Tu, Paul A. Rudnick, Misti Y. Martinez, Kristin L. Cheek, Stephen E. Stein, Robbert J. C. Slebos, Daniel C. Liebler, Depletion of abundant plasma proteins and limitations of plasma proteomics. Journal of Proteome Research. ,vol. 9, pp. 4982- 4991 ,(2010) , 10.1021/PR100646W
Majlinda Kullolli, William S. Hancock, Marina Hincapie, Automated Platform for Fractionation of Human Plasma Glycoproteome in Clinical Proteomics Analytical Chemistry. ,vol. 82, pp. 115- 120 ,(2010) , 10.1021/AC9013308
Nina Zolotarjova, Peter Mrozinski, Haiying Chen, James Martosella, Combination of affinity depletion of abundant proteins and reversed-phase fractionation in proteomic analysis of human plasma/serum. Journal of Chromatography A. ,vol. 1189, pp. 332- 338 ,(2008) , 10.1016/J.CHROMA.2007.11.082
Nina Zolotarjova, James Martosella, Gordon Nicol, Jerome Bailey, Barry E. Boyes, William C. Barrett, Differences among techniques for high-abundant protein depletion. Proteomics. ,vol. 5, pp. 3304- 3313 ,(2005) , 10.1002/PMIC.200402021
Anastasia K. Yocum, Kenneth Yu, Tomoyuki Oe, Ian A Blair, Effect of immunoaffinity depletion of human serum during proteomic investigations. Journal of Proteome Research. ,vol. 4, pp. 1722- 1731 ,(2005) , 10.1021/PR0501721