Cis-cleavage affects hammerhead and hairpin ribozyme steady-state levels differently and has strong impact on trans-targeting efficiency.
作者： Michael Andäng , Catharina Maijgren-Steffensson , Jorma Hinkula , Lars Ährlund-Richter
DOI: 10.1089/154545704322988012
关键词: Molecular biology 、 Mammalian CPEB3 ribozyme 、 Cleavage (embryo) 、 VS ribozyme 、 Hairpin ribozyme 、 Biophysics 、 Biology 、 Ribozyme 、 Hammerhead ribozyme
摘要: Trans-cleaving hammerhead or hairpin ribozymes were expressed in transgenic mice and cell lines, using a cassette containing second cis-cleaving ribozyme positioned 3′ of the trans-...
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