Novel use of COMET parameters of sperm DNA damage may increase its utility to diagnose male infertility and predict live births following both IVF and ICSI

摘要: STUDY QUESTION Do the Comet parameters of proportions sperm with low or high DNA damage improve power test in diagnosis male infertility and/or prediction IVF and ICSI live birth rates? SUMMARY ANSWER The mean score scores for were useful diagnosing provided additional discriminatory information both births. WHAT IS KNOWN ALREADY Sperm impacts adversely on fertility outcomes. DESIGN, SIZE, DURATION A retrospective study was performed involving a total 457 participants (381 patients 76 fertile donors). Data collected from clinic between 2015 2017. PARTICIPANTS/MATERIALS, SETTING, METHODS 381 consecutive partners couples attending ART donors included study. fragmentation measured by alkaline assay. Receiver operator characteristic curve analysis (area under ROC (AUC)) used to determine value average (ACS), (LCS) (HCS) diagnose factor infertility. In total, 77 226 cycles thresholds each parameter (AUC analysis) compare rates (LBRs) following ART. MAIN RESULTS AND THE ROLE OF CHANCE ACS, HCS LCS predictive (AUC > 0.9, P < 0.0001). LBRs declined once exceeded threshold levels. showed sharpest decline. Following ICSI, highest men whose levels approached range. Trends differed IVF. decreased as increased whereas but then remained stable. LIMITATIONS, REASONS FOR CAUTION Since this is first show impact births, prospective should be (stratifying based these thresholds) validate WIDER IMPLICATIONS FINDINGS Our presents novel towards elucidating genetic basis secondly relevance extent an impending success. FUNDING/COMPETING INTEREST(S) This supported Examenlab Ltd, Lister Clinic, Cryos International Imperial College London NHS Trust. No external funding obtained SL KL are employees university spin-out company commercial interest damage. other author has conflict declare. TRIAL REGISTRATION NUMBER Non-applicable.