An Optimized microRNA Backbone for Effective Single-Copy RNAi

作者: Christof Fellmann , Thomas Hoffmann , Vaishali Sridhar , Barbara Hopfgartner , Matthias Muhar

DOI: 10.1016/J.CELREP.2013.11.020

关键词: Single copyRNA interferenceMicroRNA biogenesisGeneticsSmall hairpin RNABiologyGene Knockdown TechniquesComputational biologyGenomeGene knockdownmicroRNA

摘要: … sites of the probed shRNAs were transduced at single copy with LMN vectors expressing the … -30, miR-R2, or miR-E under single-copy conditions (see Figure S1G for details). Short and …

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