Detection of disease-associated prion protein in the posterior portion of the small intestine involving the continuous Peyer's patch in cattle orally infected with bovine spongiform encephalopathy agent.

摘要: Twenty-eight calves were exposed to 5 g of homogenized brainstems confirmed as bovine spongiform encephalopathy (BSE) agents. Two five animals sequentially killed for post-mortem analyses 20 months post-inoculation (MPI) at intervals 6 or 12 months. Samples from challenged orally with BSE agents examined by Western blot and immunohistochemical analyses. Immunolabelled, disease-associated prion protein (PrPsc) was detected in a small portion follicles the continuous Peyer's patch posterior intestine involving entire ileum jejunum but not discrete patches remaining preclinical 20, 36, 48 MPI. The PrPsc-positive cells corresponded tingible body macrophages on double immunofluorescence labelling. In addition, PrPsc accumulated 7 14 central nervous system (CNS) after 34 MPI, them developed clinical signs 34, 46, 58, 66 36 MPI presented earliest detectable smallest deposition immunolabelled dorsal motor nucleus vagus nerve, spinal trigeminal medulla oblongata obex region, and/or intermediolateral 13th thoracic segment cord. Based serial killing, no CNS, including level, before 30 These results are important understanding pathogenesis BSE.