Interactions of lysophospholipids and mast cells

摘要: PHOSPHOLIPID and protein are the major components of biological membranes, much is known about their organisation in various cell membranes1,2. An important question concerns existence significance specific protein–phospholipid interactions membranes. Among methods used to elucidate these study regulatory function phospholipids intact cells. Phosphatidylserine (PS) enhances secretory response mast cell3 a highly manner. Other diacyl phospholipids, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol, phosphatidylinositol phosphatidic acid, ineffective3–5 several N-substituted derivatives PS competitive inhibitors effect on cells6. Furthermore, selectively cells IgE-dependent secretagogues7,8 without increasing IgE-independent agents such as polymyxin B9, chymotrypsin3, compound 48/80 (refs 3, 9), or A23187 (D. L. T. W. M., unpublished). As histamine secretion involves alteration membrane components10, makes it possible complex event with an established phospholipid specificity. Previously, we have fatty acid compositions11: dimyristoyl PS, dipalmitoyl distearoyl PS. We now provide evidence that lysophosphatidyl-serine (lyso-PS) generated from bovine brain by action phospholipase A2 activates induced concanavalin A (Con A) at 1/30th 1/50th concentration required for equivalent response. Although some characteristics activation lyso-PS resemble those observed its mode interaction may be different; acts micellar state12, but potentiate well below critical micelle (CMC).