Primary Separation: 2-D Electrophoresis
作者： Romina Pedreschi
DOI: 10.1007/978-1-4614-5626-1_3
关键词: Protein expression 、 Two-dimensional gel electrophoresis 、 Electrophoresis 、 Biological studies 、 Primary (chemistry) 、 Proteomics 、 Computer science 、 Computational biology 、 Pulsed-field gel electrophoresis 、 Statistical analysis
摘要: The advancements in two-dimensional electrophoresis (2DE) have consolidated it as a key tool for gel-based proteomics applications. Nowadays, 2DE is extensively applied and useful technique the simultaneous separation of hundreds to thousands proteins, analysis, differential quantification especially study nonmodel organisms. This chapter focuses on all steps involved standard protein expression. pros cons are discussed well potential variants special Special attention given data pre-processing statistical analysis generated order draw sound conclusions from biological studies.
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