High glucose inhibits receptor activator of nuclear factor‑κB ligand-induced osteoclast differentiation via downregulation of v‑ATPase V0 subunit d2 and dendritic cell‑specific transmembrane protein

作者: Juan Xu , Feng Yue , Jingbo Wang , Li Chen , Wenbo Qi

DOI: 10.3892/MMR.2014.2807

关键词: BiologyOsteoclastRANK LigandOsteoclast fusionSignal transductionActivator (genetics)RANKLDownregulation and upregulationOsteoclast maturationCell biologyMolecular biology

摘要: The balance between bone formation and resorption is compromised in diabetes, which may contribute to the high risk of fractures diabetic patients. However, mechanism by glucose affects turnover remains be elucidated. present study demonstrated that inhibited receptor activator nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis. In order examine involved inhibition osteoclastogenesis, examined several key molecules osteoclast differentiation, including v-ATPase V0 subunit d2 (Atp6V0d2), dendritic cell-specific transmembrane protein (DC-STAMP), c-fos factor activated T cells c1 (NFATc1). expression levels Atp6V0d2 DC-STAMP are regulated NFATc1 c-fos, required for fusion, important maturation. To best our knowledge, first time decreased gene ATP6v0d2 RAW264.7 mediated RANKL. Therefore, suppression pre-osteoclast or fusion essential differentiation.

参考文章(44)
Fei Xu, Ya-ping Ye, Yong-hui Dong, Feng-jing Guo, An-min Chen, Shi-long Huang, Inhibitory effects of high glucose/insulin environment on osteoclast formation and resorption in vitro Journal of Huazhong University of Science and Technology [Medical Sciences]. ,vol. 33, pp. 244- 249 ,(2013) , 10.1007/S11596-013-1105-Z
Young-Yun Kong, Hiroki Yoshida, Ildiko Sarosi, Hong-Lin Tan, Emma Timms, Casey Capparelli, Sean Morony, Antonio J. Oliveira-dos-Santos, Gwyneth Van, Annick Itie, Wilson Khoo, Andrew Wakeham, Colin R. Dunstan, David L. Lacey, Tak W. Mak, William J. Boyle, Josef M. Penninger, OPGL is a key regulator of osteoclastogenesis, lymphocyte development and lymph-node organogenesis Nature. ,vol. 397, pp. 315- 323 ,(1999) , 10.1038/16852
S. L. Teitelbaum, Bone Resorption by Osteoclasts Science. ,vol. 289, pp. 1504- 1508 ,(2000) , 10.1126/SCIENCE.289.5484.1504
Anke Dienelt, Nicole I. zur Nieden, Hyperglycemia impairs skeletogenesis from embryonic stem cells by affecting osteoblast and osteoclast differentiation. Stem Cells and Development. ,vol. 20, pp. 465- 474 ,(2011) , 10.1089/SCD.2010.0205
Natasha B Khazai, George R Beck, Guillermo E Umpierrez, Diabetes and fractures: an overshadowed association. Current Opinion in Endocrinology, Diabetes and Obesity. ,vol. 16, pp. 435- 445 ,(2009) , 10.1097/MED.0B013E328331C7EB
Y. Wittrant, Y. Gorin, K. Woodruff, D. Horn, H.E. Abboud, S. Mohan, S.L. Abboud-Werner, High d(+)glucose concentration inhibits RANKL-induced osteoclastogenesis. Bone. ,vol. 42, pp. 1122- 1130 ,(2008) , 10.1016/J.BONE.2008.02.006
Erwin F Wagner, Robert Eferl, None, Fos/AP‐1 proteins in bone and the immune system Immunological Reviews. ,vol. 208, pp. 126- 140 ,(2005) , 10.1111/J.0105-2896.2005.00332.X
Rebeca Reyes-García, Pedro Rozas-Moreno, Gema López-Gallardo, Antonia García-Martín, Mariela Varsavsky, Maria Dolores Avilés-Perez, Manuel Muñoz-Torres, Serum levels of bone resorption markers are decreased in patients with type 2 diabetes. Acta Diabetologica. ,vol. 50, pp. 47- 52 ,(2013) , 10.1007/S00592-011-0347-0
A. Grigoriadis, Z. Wang, M. Cecchini, W Hofstetter, R Felix, H. Fleisch, E. Wagner, c-Fos: a key regulator of osteoclast-macrophage lineage determination and bone remodeling. Science. ,vol. 266, pp. 443- 448 ,(1994) , 10.1126/SCIENCE.7939685
D. C. Rio, M. Ares, G. J. Hannon, T. W. Nilsen, Purification of RNA using TRIzol (TRI reagent). CSH Protocols. ,vol. 2010, ,(2010) , 10.1101/PDB.PROT5439