Establishment of Aedes (Ochlerotatus) scapularis (Diptera: Culicidae) in Mainland Florida, With Notes on the Ochlerotatus Group in the United States.

摘要: Aedes scapularis (Rondani), a widespread neotropical vector mosquito species, has been included in the fauna of Florida on basis just three larval specimens that were collected middle Keys 1945. Here, we report numerous recent collections immature and adult Ae. from multiple locations two counties southern Florida. These represent first records mainland species state since initial detection 75 yr ago. Collections both larvae adults across several years indicate is now established Broward Miami-Dade Counties. contemporary this may novel dispersal subsequent establishment events populations outside United States or reemergence undetected endemic populations. To confirm morphological identification Florida, DNA barcoding region cytochrome c oxidase subunit I gene (COI) was sequenced compared to all other Ochlerotatus Group States, specifically condolescens Dyar Knab (Diptera: Culicidae), infirmatus thelcter tortilis (Theobald) trivittatus (Coquillett) Culicidae). Molecular assays sequencing specimens. Maximum likelihood phylogenetic analysis COI ITS2 sequences place distinct clade, but unable produce clades for tortilis.