Presence of Intestinal Lectin-binding Glycoproteins on the Cell Surface of Lactobacillus acidophilus

摘要: The presence of intestinal lectin-binding glycoproteins on the cell surface Lactobacillus acidophilus group lactic acid bacteria was examined. cell-surface components were extracted with 2 m guanidine hydrochloride, and electrophoresed by SDS–PAGE followed electroblotting. Several detected blots extracts from 7 11 strains tested. distribution seemed to be specific for rather than species. β-Galactoside-specific lectin chicken intestine, then labeled horseradish peroxidase. binding assayed a ligand blotting procedure using lectin. estimated 60.2 43.0 kDa, respectively, strain L. JCM1132T, but not other strains. inhibited in lactose, indicating that specific. Presumably, interaction between bacterial cel...