Identification of Potential New Protein Vaccine Candidates through Pan-Surfomic Analysis of Pneumococcal Clinical Isolates from Adults

摘要: Purified polysaccharide and conjugate vaccines are widely used for preventing infections in adults children against the Gram-positive bacterium Streptococcus pneumoniae, a pathogen responsible high morbidity mortality rates, especially developing countries. However, these polysaccharide-based have some important limitations, such as being serotype-dependent, subjected to losing efficacy because of serotype replacement manufacturing complexity cost. It is expected that protein-based will overcome issues by conferring broad coverage independent lowering production costs. In this study, we applied “shaving” proteomic approach, consisting LC/MS/MS analysis peptides generated protease treatment live cells, collection 16 pneumococcal clinical isolates from adults, representing most prevalent strains circulating Spain during last years. The set unique proteins identified all isolates, called “pan-surfome”, consisted 254 proteins, which included protective protein antigens reported so far. search new candidates with vaccine potential, 32 were present at least 50% analyzed. We selected four them (Spr0012, Spr0328, Spr0561 SP670_2141), whose protection capacity has not yet been tested, assaying immunogenicity human sera. All induced IgM antibodies infected patients, thus indicating they could enter pipeline studies. pan-surfomic approach shows its utility discovery can elicit infectious microorganisms.