Chemical Cross-Linking in Biology

摘要: by chemical cross-linking tech­ nology has greatly intensified during the past 5 years. A number of excel­ lent, timely, and chemically oriented reviews literature have appeared (4, 68, 87). Therefore, we attempted to present a critical evaluation principles strategies that govern applica­ tion technology solutions biological problems. The use reagents as molecular rulers in determination quaternary structure oligomeric protein complexes solution membranes enjoyed wide app li cation. Another as­ pect is exploitation heterobif unctional identif y ligand-binding components complex sys­ tems. It often difficult determine how proteins function multicompo­ nent, systems. classical biochemical approach isolation characterization does not always reveal tran­ sient interactions or migratory behavior proteins, which occur natural environment