Pegylated poly-l-arginine derivatives of chitosan for effective delivery of siRNA
作者： Sang Myoung Noh , Myung Ok Park , Gayong Shim , Su Eun Han , Han Young Lee
DOI: 10.1016/J.JCONREL.2010.04.005
关键词: PEG ratio 、 Viability assay 、 In vivo 、 Polyethylene glycol 、 Small interfering RNA 、 Molecular biology 、 Drug carrier 、 Hemolysis 、 Chemistry 、 Biochemistry 、 Chitosan
摘要: For delivery of siRNA, chitosan (CS) was derivatized with poly-l-arginine (PLR) and polyethylene glycol (PEG). The formation polyplexes siRNA confirmed by gel retardation. PLR-grafted CS formed nanosized particles siRNA. showed higher cellular efficiency than did CS, pegylated PLR, or PLR. extent reduction in the expression fluorescent proteins highest following treatment cells using PLR derivatives complexes specific siRNAs. Cell viability greater populations treated CS-PLR those Hemolysis erythrocytes reduced upon conjugation CS. siRNAs via revealed little dependence on serum. Molecular imaging techniques that intratumoral administration red protein-specific significantly silenced tumor tissues vivo. These results indicate might be useful for vivo therapeutic
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