Gene-chip technology and its applications

摘要: Gene Chips are finding extensive use in animal and plant science. Generally microarrays are of two kind, cDNA or oligonucleotide. were developed at Stanford University, whereas oligonucleotide by Affymetrix. The construction of oligonucleotide on a glass slide helps to compare the gene expression level treated control samples labeling mRNA with green (Cy3) red (Cy5) dyes. hybridized chip emit fluorescence whose intensity colour can be measured. RNA can done directly indirectly. Indirect method involves amino allyle modified dUTP instead pre-labelled nucleotide. Hybridization generally occurs a minimum volume possible ensure hetroduplex formation, ten fold more DNA is spotted on slide than solutions. A confocal semi laser technologies coupled with CCD camera are used for image acquisition. For standardization, house keeping genes used in gene that not present samples. Moreover, statistical analysis (image analysis) cluster softwares have been University. gene-chip technology has many applications like analysis, signatures (molecular phenotypes) promoter regulatory element co-expression.