Ultrasensitive determination of 5-methylcytosine and 5-hydroxymethylcytosine in genomic DNA by sheathless interfaced capillary electrophoresis-mass spectrometry

作者: Fang Yuan , Xiao-Hui Zhang , Ji Nie , Hong-Xu Chen , Ying-Lin Zhou

DOI: 10.1039/C5CC10155G

关键词: Cytosine5-HydroxymethylcytosineMass spectrometry5-Methylcytosinegenomic DNAElectrophoresisChromatographyChemistryCapillary electrophoresis–mass spectrometryDNA

摘要: A newly developed sheathless interface for capillary electrophoresis–mass spectrometry, using a porous tip sprayer, was first applied for highly sensitive determination of cytosine …

参考文章(28)
Rudolf Jaenisch, Adrian Bird, Epigenetic regulation of gene expression: how the genome integrates intrinsic and environmental signals Nature Genetics. ,vol. 33, pp. 245- 254 ,(2003) , 10.1038/NG1089
Rawi Ramautar, Govert W. Somsen, Gerhardus J. de Jong, CE-MS in metabolomics. Electrophoresis. ,vol. 30, pp. 276- 291 ,(2009) , 10.1002/ELPS.200800512
Zachary D. Smith, Alexander Meissner, DNA methylation: roles in mammalian development Nature Reviews Genetics. ,vol. 14, pp. 204- 220 ,(2013) , 10.1038/NRG3354
Shinsuke Ito, Li Shen, Qing Dai, Susan C. Wu, Leonard B. Collins, James A. Swenberg, Chuan He, Yi Zhang, Tet proteins can convert 5-methylcytosine to 5-formylcytosine and 5-carboxylcytosine Science. ,vol. 333, pp. 1300- 1303 ,(2011) , 10.1126/SCIENCE.1210597
Richard D. Smith, Jose A. Olivares, Nhung T. Nguyen, Harold R. Udseth, Capillary zone electrophoresis-mass spectrometry using an electrospray ionization interface Analytical Chemistry. ,vol. 60, pp. 436- 441 ,(1988) , 10.1021/AC00156A013
Keith D. Robertson, DNA METHYLATION AND HUMAN DISEASE Nature Reviews Genetics. ,vol. 6, pp. 597- 610 ,(2005) , 10.1038/NRG1655
Maria Rowena N. Monton, Tomoyoshi Soga, Metabolome analysis by capillary electrophoresis-mass spectrometry. Journal of Chromatography A. ,vol. 1168, pp. 237- 246 ,(2007) , 10.1016/J.CHROMA.2007.02.065
Charles C. Liu, Jean-Francois Alary, Pauline Vollmerhaus, Miryam Kadkhodayan, Design, optimisation, and evaluation of a sheath flow interface for automated capillary electrophoresis-electrospray-mass spectrometry. Electrophoresis. ,vol. 26, pp. 1366- 1375 ,(2005) , 10.1002/ELPS.200410133