Evidence for a second isoform of the catalytic subunit of cAMP-dependent protein kinase.
作者： M D Uhler , J C Chrivia , G S McKnight
DOI: 10.1016/S0021-9258(18)66717-1
关键词: Protein kinase A 、 Gene isoform 、 Biology 、 Molecular biology 、 Protein subunit 、 Nucleic acid sequence 、 Complementary DNA 、 Gene 、 Messenger RNA 、 Northern blot
摘要: We have used a previously characterized mouse cDNA clone for the catalytic (C) subunit of cAMP-dependent protein kinase (Uhler, M. D., Carmichael, D. F., Lee, C., Chrivia, J. Krebs, E. G., and McKnight, G. S. (1986) Proc. Natl. Acad. Sci. U. A. 83, 1300-1304), which we designate C alpha, to isolate clones coding second isoform subunit, beta. alpha hybridize 2.4-kilobase mRNA on Northern blots whereas beta detect 4.3-kilobase mRNA. Nucleotide sequence comparison between shows that codes 91% identity with alpha. Determination levels in various tissues it is most highly expressed brain although detectable all examined. The presence two genes may explain past reports heterogeneity preparations.
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