Biosynthesis of very long-chain fatty acids (C>24) in Atlantic salmon: cloning, functional characterisation, and tissue distribution of an Elovl4 elongase.
作者： Greta Carmona-Antoñanzas , Óscar Monroig , James R. Dick , Andrew Davie , Douglas R. Tocher
DOI: 10.1016/J.CBPB.2011.02.007
关键词: Fatty acid metabolism 、 Biosynthesis 、 Complementary DNA 、 Salmo 、 Heterologous expression 、 Fatty acid 、 Amino acid 、 Biochemistry 、 Polyunsaturated fatty acid 、 Biology
摘要: The elongases of very long-chain fatty acids (Elovl) account for the rate-limiting condensation step elongation process in acid (FA) biosynthesis vertebrates. One member Elovl family, Elovl4, has been regarded as a critical enzyme vertebrates production so-called (VLC-FA), group compounds that scarcely explored fish. Here we report on cloning novel Elovl4-like elongase from Atlantic salmon (Salmo salar). Elovl4 cDNA codes putative protein containing 306 amino acids. Heterologous expression yeast demonstrated efficiently elongated saturated FAs up to 36:0, with 24:0 and 26:0 appearing preferred substrates. Additionally, effectively converted C20 C22 polyunsaturated polyenoic products C36. Tissue distribution showed mRNA transcripts are abundant eye, brain testes, suggesting that, described mammals, these tissues important metabolic sites VLC-FA. Our results discussed comparison functional analyses observed proteins other vertebrates, also investigated previously salmon.
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