Attractin Gene Deficiency in Rats Leads to Impairments in Both Activity and Spatial Learning and Memory.

摘要: Attractin (ATRN), an autosomal recessive gene that is widely distributed in the brain, involved execution of a variety brain functions and associated with certain neuropsychiatric disorders. Here, we introduce novel rat strain harboring mutation ATRN was generated by knocking ATRN-G505C via CRISPR/Cas9 system. We assessed behavioral performance these mutant knock-in rats. The G505C introduced into exon 9, synthetic primer inserted introns 8-9 for genotyping. 505th amino acid, Gly (G) residue, mutated to Cys (C) i.e., GGC TGC. Behavioral experiments showed homozygous rats spent significantly more time searching escape platform acquisition trial less target area probe Morris water maze (MWM) test traveled shorter distance open field (OFT) than wild-type In addition, Western blot analysis immunohistochemistry exhibited reduced expression brain-derived neurotrophic factor (BDNF). summary, our results indicate mutations directly lead learning memory impairments slight motor deficits. These findings provide new clues mechanism which induces neurodegenerative changes.