Genomic variability within laboratory and wild subspecies of Heligmosomoides polygyrus

作者: M.A. Abu-Madi , A.P. Reid , J.W. Lewis , W.M. Hominick

DOI: 10.1017/S0022149X00013584

关键词: Heligmosomoides polygyrusgenomic DNASubspeciesDNA–DNA hybridizationBiologyGeneticsZoologyRibosomal DNARestriction enzymeRestriction fragment length polymorphismDNA

摘要: Genomic DNA extracted from laboratory and wild subspecies of the trichostrongyle nematode Heligmosomoides polygyrus were compared using RFLP DNA/DNA hybridization techniques. Eight restriction endonuclease digests genomic two hybridized with heterologous ribosomal probes total radio-isotope labelled subspecies. H. yielded different banding patterns when probed rDNA clones in Pvu II was used as probe Hin d III digests. The remaining profiles both identical.

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