ArtinM Binding Effinities and Kinetic Interaction with Leukemia Cells: A Quartz Crystal Microbalance Bioelectroanalysis on the Cytotoxic Effect

摘要: ArtinM, a D-mannose-binding lectin from Artocarpus heterophyllus (jackfruit), interacts with N-glycosylated receptors on the surface of several cells hematopoietic origin, triggering cell migration, degranulation, and cytokine release. Because malignant transformation is often associated altered expression glycans, we evaluated interaction ArtinM human myelocytic leukemia by Quartz Cristal Microbalance (QCM) cross compared this investigation cellular responses (cytotoxicity) to binding colorimetric assay MTT, which standard method in biology. QCM analysis was able provide additional information (not possible be obtained MTT) that impact knowledge medical biology related leukemic cells. For instance, it shown association rate constant membranes (obtained QCM) varies across myeloid lines, decreasing as cytotoxic effect increases. Meanwhile no differences were observed for dissociation constants so equilibrium affinity direct function event. It supposed cytotoxicity affected time glycans way higher (the lower constant) over lines.