Cyanobacterium-Derived Extracellular Carbohydrate Polymer for the Controlled Delivery of Functional Proteins.
作者： José P. Leite , Rita Mota , Joana Durão , Sara C. Neves , Cristina C. Barrias
关键词: Carbohydrate 、 Macromolecule 、 Monosaccharide 、 Divalent 、 Cyanothece 、 Matrix (biology) 、 Extracellular polymeric substance 、 Biochemistry 、 Extracellular 、 Chemistry
摘要: The unicellular cyanobacterium Cyanothece sp. CCY 0110 is a highly efficient producer of extracellular polymeric substances (EPS), releasing up to 75% the polymer culture medium. carbohydrate released medium (RPS) was previously isolated and characterized; it composed nine different monosaccharides including two uronic acids, also containing peptides sulfate groups. Here shown that RPS spontaneously assembles with proteins at high concentrations leading phase transition. are progressively structurally intact near physiological conditions, primarily through swelling polymer-protein matrix. kinetics can be modulated addition divalent cations, such as calcium. Notably, not toxic human dermal neonatal fibroblasts in vitro bellow 0.1 mg mL-1 . results show this good candidate for delivery therapeutic macromolecules.
sci-hub.se 参考文章(31)
Misu Moscovici, Present and future medical applications of microbial exopolysaccharides. Frontiers in Microbiology. ,vol. 6, pp. 1012- 1012 ,(2015) , 10.3389/FMICB.2015.01012
Marya Ahmed, Ravin Narain, Carbohydrate-based materials for targeted delivery of drugs and genes to the liver Nanomedicine: Nanotechnology, Biology and Medicine. ,vol. 10, pp. 2263- 2288 ,(2015) , 10.2217/NNM.15.58
Sara Pereira, Andrea Zille, Ernesto Micheletti, Pedro Moradas-Ferreira, Roberto De Philippis, Paula Tamagnini, Complexity of cyanobacterial exopolysaccharides: composition, structures, inducing factors and putative genes involved in their biosynthesis and assembly Fems Microbiology Reviews. ,vol. 33, pp. 917- 941 ,(2009) , 10.1111/J.1574-6976.2009.00183.X
Monica C. Branco, Darrin J. Pochan, Norman J. Wagner, Joel P. Schneider, The Effect of Protein Structure on their Controlled Release from an Injectable Peptide Hydrogel Biomaterials. ,vol. 31, pp. 9527- 9534 ,(2010) , 10.1016/J.BIOMATERIALS.2010.08.047
Raj Kumar Shukla, Akanksha Tiwari, Carbohydrate polymers: Applications and recent advances in delivering drugs to the colon Carbohydrate Polymers. ,vol. 88, pp. 399- 416 ,(2012) , 10.1016/J.CARBPOL.2011.12.021
Huub Schellekens, Bioequivalence and the immunogenicity of biopharmaceuticals Nature Reviews Drug Discovery. ,vol. 1, pp. 457- 462 ,(2002) , 10.1038/NRD818
Yusuke Nagai, Larry D. Unsworth, Sotirios Koutsopoulos, Shuguang Zhang, Slow release of molecules in self-assembling peptide nanofiber scaffold. Journal of Controlled Release. ,vol. 115, pp. 18- 25 ,(2006) , 10.1016/J.JCONREL.2006.06.031
Christian Geraths, Laura Eichstädter, Raphael J. Gübeli, Erik H. Christen, Christian Friedrich, Wilfried Weber, Synthesis and characterization of a stimulus-responsive l-ornithine-degrading hydrogel Journal of Controlled Release. ,vol. 165, pp. 38- 43 ,(2013) , 10.1016/J.JCONREL.2012.10.022
Changhoon Chai, Jooyoung Lee, Qingrong Huang, None, The effect of ionic strength on the rheology of pH-induced bovine serum albumin/κ-carrageenan coacervates LWT - Food Science and Technology. ,vol. 59, pp. 356- 360 ,(2014) , 10.1016/J.LWT.2014.05.024
Qiaomei Ru, Yuwen Wang, Jooyoung Lee, Yuting Ding, Qingrong Huang, Turbidity and rheological properties of bovine serum albumin/pectin coacervates: Effect of salt concentration and initial protein/polysaccharide ratio Carbohydrate Polymers. ,vol. 88, pp. 838- 846 ,(2012) , 10.1016/J.CARBPOL.2012.01.019