Development of a Standard Test To Assess the Resistance of Staphylococcus aureus Biofilm Cells to Disinfectants
作者： Suzanne B. I. Luppens , Martine W. Reij , Rob W. L. van der Heijden , Frank M. Rombouts , Tjakko Abee
DOI: 10.1128/AEM.68.9.4194-4200.2002
关键词: Sodium hypochlorite 、 Microbiology 、 Oxidizing agent 、 Hypochlorous acid 、 Biofilm 、 Staphylococcus aureus 、 Disinfectant 、 Biology 、 Hypochlorite 、 Benzalkonium chloride
摘要: A standardized disinfectant test for Staphylococcus aureus cells in biofilms was developed. Two disinfectants, the membrane-active compound benzalkonium chloride (BAC) and oxidizing agent sodium hypochlorite, were used to evaluate biofilm test. S. formed on glass, stainless steel, polystyrene a simple system with constant nutrient flow that mimicked as closely possible conditions current standard European (EN 1040). The glass contained cell clumps extracellular polysaccharides. average surface coverage 60%, most (92%) of viable. Biofilm formation disinfection different experiments reproducible. For exposed BAC hypochlorite concentrations needed achieve 4-log killing 50 600 times higher, respectively, than this level phase 1 suspension cells. Our results show is useful addition tests.
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