作者: Darlene Hartze , Kathleen A. Hickey , Douglas Peterson , John R. Gillis
DOI:
关键词: Lethality 、 Steam temperature 、 Absorption (chemistry) 、 Spore 、 Spectrum analyzer 、 Analytical chemistry 、 Incubation period 、 Biological evaluation 、 Contamination 、 Chemistry 、 Chromatography
摘要: Multiple-component integral-structure dual-test sterility indicator, method of assembling physical components, selected spores, and chemical constituents in solution, within the indicator arranged to enable dual-evaluations bacterial-lethality a dry-goods load, resulting from exposure saturated-steam sterilizing cycle. The are formulated chemically produce reaction-product, which is quantitatively responsive combined effect cycle steam temperature time at that temperature, for providing promptly-available spectroscopic evaluation bacterial-lethality. Absorption narrow-band visible-light wavelength by if any, chemical-constituent used measure combined-effect exposure. Subsequent such prompt-response bacterial-lethality, biological utilizing confined contents same test free risk ambient contamination any contents, evaluating pH change, due live spore-growth, or absence thereof, following predetermined spore incubation period, exposed liquid constituent-solution spore-culturing evaluation.