8-Hydroxydeoxyguanosine and 8-hydroxyguanine as biomarkers of oxidative DNA damage.
作者： Harold J. Helbock , Kenneth B. Beckman , Bruce N. Ames
DOI: 10.1016/S0076-6879(99)00123-8
关键词: DNA extraction 、 Oxidation reduction 、 Oxidative dna damage 、 Deoxyguanosine 、 8-Hydroxy-2'-deoxyguanosine 、 DNA damage 、 8-hydroxydeoxyguanosine 、 Chemistry 、 Biochemistry 、 DNA oxidation
摘要: Publisher Summary Modifications and alternatives to the original HPLC-EC (high-performance liquid chromatography- electrochemical) methods have produced a burgeoning literature on oxidative DNA damage. At same time, they resulted in discordant results disagreements about most appropriate techniques. Because of apparent importance damage, interest 8-Oxo-2'-deoxyguanosine (oxo 8 dG) oxo Gua as biomarkers for this it is important identify best available assay methods. This chapter reviews that find be reliable, indicate common pitfalls associated with these techniques, uncertainties interpretation. Methods isolation continue improve, minor modification chaotropic NaI technique producing lowest dG values reported date using HPLC-EC. Although use urinary adducts monitor oxidation vivo remains attractive, can difficult interpret.
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