Direct measurement of effective diffusion coefficients in nanochannels using steady-state dispersion effects

摘要: We present a method to measure effective diffusion coefficients of fluorescently labeled molecules inside nanofluidic system. Molecules with small show larger lateral dispersion than highly diffusive species, which is counterintuitive. performed measurements wheat germ agglutinin proteins and obtained an coefficient four orders magnitude lower its free coefficient. Our technique direct relatively simple measurement the nanochannels well controlled dimensions could help fundamental studies in membranes separation sciences.