Mechanobiology Assays with Applications in Cardiomyocyte Biology and Cardiotoxicity.

摘要: Cardiomyocytes are the motor units that drive contraction and relaxation of heart. Traditionally, testing drugs for cardiotoxic effects has relied on primary cardiomyocytes from animal models focused short-term, electrophysiological, arrhythmogenic effects. However, present challenges arising their limited viability in culture, tissue suffers a mismatch physiology to human heart muscle. Human-induced pluripotent stem cell-derived (hiPSC-CMs) can address these challenges. They also offer potential study not only electrophysiological but changes cardiomyocyte contractile mechanical function response drugs. With growing recognition long-term some subcellular structure function, there is increasing interest using hiPSC-CMs vitro cardiotoxicity studies. This review provides brief overview techniques be used quantify active force generate variations inherent stiffness It concludes by discussing application tools understanding how directly impact mechanobiology sense respond load at cellular level.