Development of a qPCR assay for specific quantification of Botrytis cinerea on grapes.
作者： Camélia Filofteia Diguta , Sandrine Rousseaux , Stéphanie Weidmann , Nicolas Bretin , Béatrice Vincent
DOI: 10.1111/J.1574-6968.2010.02127.X
关键词: Reverse transcription polymerase chain reaction 、 DNA extraction 、 Ribosomal DNA 、 Intergenic region 、 Botrytis cinerea 、 Polymerase chain reaction 、 Biology 、 Botrytis 、 Real-time polymerase chain reaction 、 Molecular biology
摘要: The aim of this study was to develop a system for rapid and accurate real-time quantitative PCR (qPCR) identification quantification Botrytis cinerea , one the major pathogens present on grapes. intergenic spacer (IGS) region nuclear ribosomal DNA used specifically detect quantify B. . A standard curve established fungus. qPCR reaction based simultaneous detection specific IGS sequence also contained an internal amplification control compensate variations in extraction various compounds from grapes that inhibit PCR. In these conditions, assay had high efficiency (97%), limit estimated be 6.3 pg (corresponding 540 spores). Our method applied assess effects treatment strategies against vineyard. proved rapid, selective sensitive may monitor infection vineyards.
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