Immobilization of commercial laccase onto green coconut fiber by adsorption and its application for reactive textile dyes degradation

作者: Raquel O. Cristóvão , Ana P.M. Tavares , Ana Iraidy Brígida , José M. Loureiro , Rui A.R. Boaventura

DOI: 10.1016/J.MOLCATB.2011.04.014

关键词: LaccaseBiocatalysisEffluentChemical engineeringEnzyme actionContact timeChemistryAdsorptionSewage treatmentImmobilized enzymeChromatography

摘要: Abstract An effort has been made to find a cheaper, easily available and simple alternative for the immobilization of enzymes subsequent utilization at large scale in textile wastewater treatment. Commercial laccase was immobilized first time on an agroindustrial residue, green coconut fiber, by physical adsorption. The effect conditions (enzyme concentration, contact pH value) properties biocatalyst determined. Then, enzyme characterization performed kinetic parameters were obtained. Thermal operational stabilities improved compared with free commercial showing its potential continuous applications. Finally, performance degradation various reactive dyes mixture them batch reactors evaluated. Two phenomena observed: decolourization solutions due adsorption support action. A high percentage practically all two cycles effective dye obtained, suitability colour removal from industrial effluents.

参考文章(38)
Paul Ander, Kurt Messner, Oxidation of 1-hydroxybenzotriazole by laccase and lignin peroxidase Biotechnology Techniques. ,vol. 12, pp. 191- 195 ,(1998) , 10.1023/A:1008813206178
Y.M. Slokar, A. Majcen Le Marechal, Methods of decoloration of textile wastewaters Dyes and Pigments. ,vol. 37, pp. 335- 356 ,(1998) , 10.1016/S0143-7208(97)00075-2
P.-P. Champagne, J.A. Ramsay, Reactive blue 19 decolouration by laccase immobilized on silica beads Applied Microbiology and Biotechnology. ,vol. 77, pp. 819- 823 ,(2007) , 10.1007/S00253-007-1208-1
Jian Yu, Xiaowei Wang, PL Yue, Optimal Decolorization and Kinetic Modeling of Synthetic Dyes by Pseudomonas Strains Water Research. ,vol. 35, pp. 3579- 3586 ,(2001) , 10.1016/S0043-1354(01)00100-2
Uwe T. Bornscheuer, Immobilizing Enzymes: How to Create More Suitable Biocatalysts Angewandte Chemie International Edition. ,vol. 42, pp. 3336- 3337 ,(2003) , 10.1002/ANIE.200301664
Susan Davis, RichardG. Burns, Covalent immobilization of laccase on activated carbon for phenolic effluent treatment Applied Microbiology and Biotechnology. ,vol. 37, pp. 474- 479 ,(1992) , 10.1007/BF00180972
Müfrettin Sarı, Sinan Akgöl, Melike Karataş, Adil Denizli, Reversible Immobilization of Catalase by Metal Chelate Affinity Interaction on Magnetic Beads Industrial & Engineering Chemistry Research. ,vol. 45, pp. 3036- 3043 ,(2006) , 10.1021/IE0507979
Linqiu Cao, Immobilised enzymes: science or art? Current Opinion in Chemical Biology. ,vol. 9, pp. 217- 226 ,(2005) , 10.1016/J.CBPA.2005.02.014
Raquel O. Cristóvão, Ana P.M. Tavares, Luísa A. Ferreira, José M. Loureiro, Rui A.R. Boaventura, Eugénia A. Macedo, Modeling the discoloration of a mixture of reactive textile dyes by commercial laccase. Bioresource Technology. ,vol. 100, pp. 1094- 1099 ,(2009) , 10.1016/J.BIORTECH.2008.08.007