Parametric continuous feed stream design to fine‐tune fed‐batch bioreactor performance: recombinant human growth hormone production in Bacillus subtilis
作者： Güzide Çalık , Pınar Kocabaş , Hande Afşar , Pınar Çalık , Tunçer H Özdamar
DOI: 10.1002/JCTB.4864
关键词: Biotechnology 、 Fermentation 、 Food science 、 Chemistry 、 Bacillus subtilis 、 Transition time 、 Recombinant DNA 、 Human growth hormone 、 Fed batch bioreactor 、 Bioreactor 、 Production (economics)
摘要: BACKGROUND Design principles of a fed-batch bioreactor system to fine-tune performance by increasing production and productivity are investigated. To enhance the in fermentation recombinant human growth hormone (rhGH) Bacillus subtilis, this work develops continuous feed stream (CFS) design method with parameters, cultivation time switch batch- operation, so called transition time, tT, specific rate, μ. RESULTS Based on batch-bioreactor data for rhGH B. subtilis (scoC−) carrying pMK4::pre(subC)::hGH, three components defined medium were determined feeding. Theoretical analysis modelling reactor lead CFS design, be applied starting at which is function μ. Transition tT was optimised experimentally using batch operation μ value, 0.100 h−1; highest concentration obtained tT=4 h. increased further 127 mg dm−3, when flow rate calculated μ = 0.125 h−1. CONCLUSION The process enhanced 36% 8 h shortened t = 16 h, 2.4-fold compared batch-fermentation. © 2015 Society Chemical Industry
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