Voltage- and Ca(2+)-gated currents in zebrafish olfactory receptor neurons.
作者： F S Corotto , W C Michel , N Chen , D R Piper
DOI:
关键词: Conductance 、 Anatomy 、 Receptor potential 、 Tetrodotoxin 、 Biophysics 、 Zebrafish 、 Olfactory receptor 、 Depolarization 、 Voltage 、 Time constant 、 Chemistry
摘要: Voltage- and Ca(2+)-gated currents were recorded from isolated olfactory receptor neurons (ORNs) of the zebrafish Danio rerio using whole-cell voltage-clamp technique. Zebrafish ORNs had an average capacitance 0.66 pF apparent input resistance 8.0 G omega. Depolarizing steps elicited transient inward followed by outward with sustained components. The current (INa) was sensitive to 1 mumol l-1 tetrodotoxin, activated between -74mV -64mV, reached half-maximal conductance at -28 mV. Its peak amplitude averaged -101pA. Steady-state inactivation INa test potential -78mV recovery proceeded two time constants averaging 23 ms 532 ms. A sustained, Co(2+)-sensitive (ICa) -44mV -34mV a -9pA -14mV. Outward carried K+, based on reversal potentials tail currents, consisted Ca(2+)-dependent K+ current, delayed rectifier (IDR) (IA). (IK(Ca)) -34mV, whereas IDR IA -24mV. In summary, possess complement gated similar but not identical that other vertebrates which appears well suited for encoding graded into train action potentials.
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