Human red cell acid phosphatase (ACP1). The amino acid sequence of the two isozymes Bf and Bs encoded by the ACP1*B allele.

摘要: The pair of isozymes, Bf and Bs, encoded by the human red cell acid phosphatase ACP1*B allele has been sequenced. Similar but not identical primary structures were observed. Both isozymes consist a single peptide chain 157 amino residues, which is acetylated at amino-terminal alanine residue. Bs are glycosylated, calculated molecular masses 17,932 17,867 Da, respectively. They except for sequence segment 40-73, peculiar to respective isozyme. This consistent with our hypothesis that two generated as result alternative splicing RNA transcript. finding signature offers basis characteristic differences in catalytic properties isozymes. A high degree homology was found between isozyme 18-kDa cytosolic from bovine liver. No observed other sequenced proteins, this establishes these low weight phosphatases products distinct gene family.