Androgens and androgenic activity in broiler manure assessed by means of chemical analyses and in vitro bioassays

摘要: The use of manure as an agricultural amendment is increasing the release steroid hormones into environment. Most research in this field has focused on estrogenic phenomena, with less attention paid to androgenic substances. present study assessed activity broiler using vitro approaches based cells stably transfected androgen receptor. Leaching experiments were also performed observe whether endocrine disruptors pass through a soil column and potentially reach groundwater. In parallel, analytical chemistry method was used determine contribution most important natural androgens androgenicity. Samplings at 4 farms 2 seasons. All but samples showed activity. leakage experiments, however, no detectable leachates or soils after leaching. According results, androgenicity can be attributed mainly (but not completely) androstenedione, dihydrotestosterone. Similarly bioassays, chemical analysis did reveal presence any soils. These results point rapid degradation substances responsible for under experimental conditions study. However, long-term effects associated constant intensive application land require further attention. Environ Toxicol Chem 2017;36:1746-1754. © 2016 SETAC.