Label-free colorimetric assay for biological thiols based on ssDNA/silver nanoparticle system by salt amplification.
作者： Zhang Chen , Yejuan He , Shenglian Luo , Hailan Lin , Yufang Chen
DOI: 10.1039/B925683K
关键词: Conjugated system 、 Detection limit 、 Homocysteine 、 Silver nanoparticle 、 Nanoparticle 、 Cysteine 、 Glutathione 、 Inorganic chemistry 、 Selectivity 、 Chemistry 、 Nuclear chemistry
摘要: We report a novel method for biological thiols detection using ssDNA/sliver nanoparticles system. The adsorbing ssDNA supplies silver high density charge to rescue from aggregation induced by salt. However, homocysteine (cysteine or glutathione) is conjugated more powerfully than AgNPs via Ag–S bond, which holds back binding surface. When salt added, occurs and the corresponding color changes yellow brown after these introduced. A sensitivity can be achieved as an amplifier assay thiols. In our study, favorable linear correlation between A0/Ax ratio concentration was obtained in range of 10 500 nM with low limit nM, indicating that could analyzed at concentration. 300 caused visible change. As well as, cysteine glutathione detected 50 100 respectively. addition, study on selectivity this shows only homocysteine, generate signal response.
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