Non-Random Chromosome Changes Involving the Distal End of Chromosome 15 and Chromosomes 6 and 12 in Pristane-Induced Mouse Plasmacytomas

摘要: The karyotypes of 7 pristane induced mouse plasmacytomas were studied by G-banding. Only primary tumors or early passage generations analyzed. All showed a consistent translocation the distal part chromosome No. 15 either to 6 (rcpT (6; 15) 12 (T(12;15)). specific breakpoints at 6C, 15D3/E and 12F2. Early often mixed population with two different translocations. Considered together known karyotypic features murine human lymphomas, these findings support theory that non-random chromosomal changes in lymphoproliferative malignancies are associated type target cell, rather than etiological agent. Moreover, involvement chromosomes carry heavy chain (No. 12) light 6) determinants respectively, raises question whether translocations may be related rearrangements DNA level occur during differentiation normal plasma cells. Following discovery 1960 association Philadelphia (Ph') chronic myelogenous leukemia (CML),27 much attention has been focused on cytogenetic other neoplasms immunohematopoietic tissues. development staining techniques which lead identification greatly expanded this field.4 Ph' arose from segment 22 end long arm 9.33 This is not thought marker for leukemic cells, as it found also hematopoietic cells patients CML. However Ph’ considered clonal an abnormal stem cell. Derivatives clone have proliferative disorders affecting series: hyperplastic granulocytes late blastic cells. described 4 cases myeloma among 70 studies Van DenBerghe et al.45 Two had carried chromosomes. The search abnormality multiple yet produced such straightforward Ph'. Cytogenetic myeloma, before availability banding techniques, indicated various forms aneuploidy, but even all cases.28 Using Giemsa staining, Wuster-Hill al.50 14 cell myeloma. Liang Rowley20 14q+ 3 1 studied. Moreover tl4q+ B-cell including Burkitts Lymphoma,8,21,23,55 rarely non lymphomas11 follicular lymphoma.5 peripheral blood lymphocytes 8 hereditary ataxia-telangiectasia23 marker. Zech al.55 extra band was probably derived 8. These suggest B-lymphocytes frequently involving 14. Cytogenetic mineral-oil BALB/c mice, completed individual banding, demonstrated high frequency polyploidy-aneuploidy, chromosomes.54 Transplanted used work difficult identify change tumor development. Later, Yosida al.52 only diploid tumors, rest modal numbers between tripoid tetraploid number. changed near tetraploidy transplantation. Moriwaki al.25 followed one (MSPC-1) through 40 transfer repeatedly gave rise variants. cause polyploidy explained. Banding analysis long-term transplanted vitro explanted lines 38,39,53 presence deleted T (12; possibly (10; 15).56 Shepard al. reported plasmacytoma MOPC-31C. al.41 5 term MOPC21, MOPC315, MOPC31C, MSPC-1 X5563 15-chromosomes common breakpoint D31E. Further, translocated chromosomes. or, characteristic, does important association. Very recently Manolova al.22 new (Mar 17p+) consecutive myeloma. Plasmacytomas rapidly progressing pass quickly stages increasing autonomy time diagnosis their establishment growing transplantable tumors. For example, mineral oil pristane-induced require special conditions growth; most hosts do grow when transferred intraperitoneally however injected into previously treated intraperitoneal pristane, they virtually 100% recipients.3,30 Plasmacytomas can detected smears ascites. In number induction progress our laboratory, we noted different, morphological forms. Some small close size while others (including transplants) typical (and tetraploid) large cells. To further associate origin exclude artefacts incurred transplantation, analyzed constitution primary, first second mostly “small cell” type. Much reported.27a Chromosomal involved 6, 15. appearance immunological interest because, mouse, shown structural genes Ig-heavy chains11,15 kappa L-chain genes.43 Croce al.9 chains located 14. In earlier highly 15-trisomy found, irrespective leukemia-inducing agents included Gross, RadLV Moloney viruses, X-rays chemical carcinogens.7,10,47,48 Recently, critical area T6 15.49