Activating and Elucidating Metabolism of Complex Sugars in Yarrowia lipolytica.

摘要: The oleaginous yeast Yarrowia lipolytica is an industrially important host for production of organic acids, oleochemicals, lipids, and proteins with broad biotechnological applications. Albeit known decades, the unique native metabolism Y. using complex fermentable sugars, which are abundant in lignocellulosic biomass, poorly understood. In this study, we activated elucidated sugar cell growth on xylose cellobiose as well their mixtures glucose through comprehensive metabolic transcriptomic analyses. We identified 7 putative glucose-specific transporters, 16 xylose-specific 4 cellobiose-specific transporters that transcriptionally upregulated respective single sugars. capable a carbon source, but dehydrogenase key bottleneck assimilation repressed by glucose. has set 5 extracellular 6 intracellular β-glucosidases assimilating via extra- mechanisms, latter being dominant sole source. Strikingly, exhibited enhanced utilization mixed strong catabolite activation mixture mild repression cellobiose. results study shed light fundamental understanding will help guide inverse engineering conversion biomass-derived sugars to chemicals fuels.