Mosquito feeding modulates Th1 and Th2 cytokines in flavivirus susceptible mice: an effect mimicked by injection of sialokinins, but not demonstrated in flavivirus resistant mice

摘要: Culex pipiens and Aedes aegypti mosquitoes were fed on C3H/HeJ mice systemic cytokine production was quantified from stimulated lymphocytes harvested four to ten days after feeding. Mosquito feeding significantly down regulated IFN gamma seven post by Cx. Ae Th2 cytokines, IL-4 IL-10, up 4-7 Ae. The immunosuppressive effect of not evident in congenic flavivirus resistant (C3H/RV) mice, as IL-2 at 7 10, correlating with a significant decrease 10 mosquitoes. Inoculation 5-1000 ng sialokinin-I into mimicked the regulating Th1 cytokines inoculation. Injections sialokinin-II resulted only moderate effects injection. Thus natural two arbovirus vectors had profound T cell modulatory vivo virus susceptible animals which demonstrated host. Moreover, mosquito modulating host response. These results may lend new insight specific aspects role vector potentiating transmission mammalian