Characterization of a Marine Bacterium Associated with Crassostrea virginica (the Eastern Oyster)

作者: Ronald M. Weiner , Anca M. Segall , Rita R. Colwell

DOI: 10.1128/AEM.49.1.83-90.1985

关键词: BotanyEastern oysterPigmentBacteriaMolecular massBiologyMolluscaOysterMelaninCrassostreaMicrobiology

摘要: A gram-negative bacterium found to be closely associated with oysters has been isolated and characterized. The organism, designated LST, a generation time of 106 min in Marine broth under optimal growth conditions at 25°C. During the decline phase growth, it exhibits morphological transition from motile rod (ca. 1 μm length) an elongated, 3- 40-μm, nonmotile, tightly coiled helix. LST synthesizes releases pigment stationary phases growth. Identified as melanin on basis chemical properties UV absorbance maxima, comprises polymers heterogeneous molecular weights, ranging 12,000 120,000. guanosine-plus-cytosine content DNA is 46%, results phenetic analysis DNA-DNA hybridization indicate that this represents new species. adheres variety surfaces, including glass, plastics, oyster shell, shown promote settlement larvae. Images

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