Metabolic and metagenomic profiling of hydrocarbon-degrading microorganisms obtained from the deep biosphere of the Gulf of Mexico

摘要: Abstract Marine microbes are capable of degrading hydrocarbons; however, those inhabiting the deep biosphere (>1000 m) remain largely unexplored. Microbial metabolism could lead to generation diverse chemistries (some with therapeutic activities), but impact using hydrocarbons as sole source microbial energy in synthesis metabolites, remains obscure. Here, we investigated metagenomic and metabolomic profile two deep-marine sediments (>1 200 m deep, designated A7 B18) collected from Gulf Mexico (GM) when grown for 28 days a simple mixture 4 complex hydrocarbon (petroleum API 40) their energy. State art techniques analysis (e.g., Global Natural Products Social Molecular Networking, network annotation propagation [NAP], MS2LDA) were used describe associated utilization hydrocarbons. The sequencing suggests predominant abundance Proteobacteria environmental 40-enriched samples, while Pseudomonas increased after growth 40. presence predominantly lipid lipid-like phenyl propanoids polyketides superclass (Classyfire annotation). Hydrocarbon derivatives detected carboxylic acids azelaic sebacic acid) or alcohols, non-hydrocarbon related also including tetracycline-related metabolites sphinganines. Our study provides valuable chemical microbiological information one most understudied ecosystems earth, marine biosphere.