作者: Narayana Annaluru , Héloïse Muller , Sivaprakash Ramalingam , Karthikeyan Kandavelou , Viktoriya London
DOI: 10.1007/978-1-61779-564-0_7
关键词: Biology 、 DNA synthesis 、 Insert (molecular biology) 、 DNA 、 Gene 、 Base pair 、 Recombinant DNA 、 DNA polymerase 、 Computational biology 、 Biochemistry 、 Plasmid
摘要: … Here, we describe rapid and efficient assembly of multiple DNA fragments using the USER-mediated approach (also known as USER fusion) for both in vitro (using Phusion polymerase …