Assembling DNA fragments by USER fusion.

作者: Narayana Annaluru , Héloïse Muller , Sivaprakash Ramalingam , Karthikeyan Kandavelou , Viktoriya London

DOI: 10.1007/978-1-61779-564-0_7

关键词: BiologyDNA synthesisInsert (molecular biology)DNAGeneBase pairRecombinant DNADNA polymeraseComputational biologyBiochemistryPlasmid

摘要: … Here, we describe rapid and efficient assembly of multiple DNA fragments using the USER-mediated approach (also known as USER fusion) for both in vitro (using Phusion polymerase …

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