Use of the monoclonal antibody 12F1 to characterize the differentiation antigen VLA-2.

摘要: A monoclonal antibody, 12F1, has been produced that specifically immunoprecipitates the human cell surface structure VLA-2 from platelets and long-term activated T cells, as well fibroblast neuroblastoma lines. Cross-linking studies indicate exists on a 165,000 Mr heavy chain (alpha 2) in noncovalent 1:1 association with 130,000 light (beta). The antibody A-1A5, which reacts beta subunit common to all VLA structures, was able completely preclear VLA-2, indicating of alpha 2 associated beta-chain. specificity 12F1 for allowed independent immunoprecipitation flow cytometry analysis this separate any other structures may have present such VLA-1 or free beta-subunit. Subunit dissociation were used demonstrate recognizes an epitope is consistent alone among proteins. Analysis cells indicated like VLA-1, another "very late" appearing activation antigen arises concurrently starting at day 7 increasing through wk. found many same (inactivated leukemia fibroblasts, SK-N-SH cells), but can be expressed independently, because also VLA-1-negative HSB platelets, VLA-2-negative C8215 cells.