Comparing passive dosing and solvent spiking methods to determine the acute toxic effect of pentachlorophenol on Daphnia magna

摘要: Pentachlorophenol (PCP) is a widespread and persistent hydrophobic organic pollutant in the environment despite its restricted public use. Risk assessment of such compounds (HOCs) challenging because sorption volatilization issues during toxicity test often lead to inconsistent exposure concentration. Considering hydrophobicity PCP, this study, passive dosing format was applied by adopting silicone O-ring as reservoir evaluated applicability on determination PCP Daphnia magna. Results obtained with method were compared that solvent spiking method. We hypothesized may provide more reliable accurate results than conventional approach. As result, partition coefficient between methanol medium (log KMeOH:ISO) 2.1, which enabled maintenance concentration throughout experiment. In acute tests, showed similar EC50 values 576 485 µg/L for 24 h, 362 374 µg/L 48 h, respectively, overlap previous studies. Altogether, both methods suitable PCP.