Minor Antigens on Transfused RBCs Crossprime CD8 T Cells but Do Not Induce Full Effector Function

摘要: HLA-matched bone marrow transplantation (BMT) is a cure for nonmalignant hematological disorders; however, rejection rates are high and correlate with the number of antecedent transfusions. Recently, using murine models, we reported that minor antigens (mHAs) in transfused leukoreduced red blood cell (RBC) or platelet units induce subsequent BMT. To study RBCs as an immunogen, utilized transgenic donors express model mHA selectively on (HOD mouse). Transfusion HOD did not BMT shared mHAs RBCs. Similarly, no endogenous anti-HOD CD8+ T-cell response was detected antigen-specific tetramer reagents. Adoptively transferred OT-I T cells rapidly expanded after transfusion; only semi-effector phenotype observed (tumor necrosis factor-α interferon-γ secretion, but essentially Granzyme B). After initial expansion, contracted to very low levels. A similar trend by in vivo CTL assay, transient lytic activity. Together, these data indicate may be component RBC induces rejection, suggest contaminating platelets leukocytes responsible.