Rapid short-column chromatography of amino acids

作者: Vivian E. Shih , Mary L. Efron , Gerald L. Mechanic

DOI: 10.1016/0003-2697(67)90035-8

关键词: Column chromatographyBiochemistryMetabolic diseaseChromatographyHomocystineAmino acid metabolismPhenylalanineUrineBiologyTyrosineAmino acidBiophysicsCell biologyMolecular biology

摘要: Abstract Methods are described for the rapid analysis of certain individual amino acids (e.g., homocystine) and groups branched-chain acids), which relevant to particular metabolic diseases. All methods use ion-exchange chromatography on a 20 × 0.9 cm column. These were developed especially clinical application in diagnosis therapy inborn errors acid metabolism. analyses completed less than 2.5 hr.

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