Autologous Down-Regulation of Messenger Ribonucleic Acid and Protein Levels for Estrogen Receptors in MCF-7 Cells: An Inverse Correlation to Progesterone Receptor Levels*
作者： A. H. REE , B. F. LANDMARK , W. ESKILD , F. O. LEVY , H. LAHOOTI
关键词: Estrogen 、 Internal medicine 、 Estrogen receptor 、 Endocrinology 、 Progesterone receptor 、 Cell culture 、 MCF-7 、 Receptor 、 Downregulation and upregulation 、 Messenger RNA 、 Biology
摘要: In the present study we have examined effects of estradiol on mRNA levels for estrogen (ER) and progesterone receptors (PR) in estrogen-dependent mammary carcinoma MCF-7 cell line. The changes ER immunoactivity specific binding [3H]R5020 were also assessed. Estradiol (10-7 M) caused a transient time-dependent reduction level ER, with maximal effect (30-40% control; n = 3) after 72 h. This was associated similar decrease immunoactivity. Further treatment (96 120 h) revealed return to control values, whereas remained depressed. PR gave almost inverse curve. Initially (24-72 h), observed pronounced increase this mRNA, (6-7 times value; Treatment beyond h gradual toward level. variation changes, except tha...
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