A novel animal model for dry mouth: E2f1-deficient NOD/SCID mice

摘要: Abstract It is well known that hyposecretion of saliva and consequent dry mouth can lead to severe dental caries, periodontal disease, mucosal infections. To investigate the mechanisms hyposalivation, a animal model required. Recently, we established E2f1-deficient non-obese diabetic/severe combined immunodeficiency disease (NOD/SCID.E2f1 −/− ) mice. In this article, review NOD/SCID.E2f1 mice, which are convenient in vivo for mouth. Behavioral analyses mice revealed they increased water consumption when ate food, frequency time these spent on intake were higher longer than observed control volume secreted stimulated with secretagogue was less Histological salivary glands had more ducts Immunohistochemical acini localization aqaporin-5 (AQP5), channel involved fluid secretion, differed compared mice; AQP5 leaky diffusively localized from apical membrane cytosol Covalent modification by ubiquitination detected suggesting changes acinar/duct structure down-regulation gland caused pathogenesis hyposalivation Furthermore, mutant did not have any underlying diseases, such as diabetes. Therefore may provide useful